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Registros recuperados: 94 | |
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Vicari,Marcelo Ricardo; Artoni,Roberto Ferreira; Bertollo,Luiz Antonio Carlos. |
The chromosomes of specimens from four Hoplias malabaricus populations from headwaters of adjacent river basins at Ponta Grossa, southern Brazil, were investigated using differential staining techniques (C-banding, AgNO3 and CMA3) and fluorescent in situ hybridization (FISH) with an 18S rDNA probe. The diploid chromosome number in representatives of all four populations was invariably 2n = 42, with karyotypes composed of 12 pairs of metacentrics and 9 pairs of submetacentrics, without heteromorphic sex chromosomes. This kind of karyotype represents cytotype A in regard to cytotypes identified previously in H. malabaricus, exhibiting however, at the same time, some differences in the distribution of constitutive heterochromatin segments and in the locations... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome banding; FISH; Cytotaxonomy of trahiras; Karyotype; NOR phenotype. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100018 |
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Silva,Guilherme Messias da; Bione,Edgar Guimarães; Cabral-de-Mello,Diogo Cavalcanti; Moura,Rita de Cássia de; Simões,Zilá Luz Paulino; Souza,Maria José de. |
Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-submetacentric chromosomes. The sex determination mechanism was of the Xy p type in D. nisus and D. semisquamosus and of the Xyr type in D. sericeus. C-banding revealed the presence of pericentromeric blocks of constitutive heterochromatin (CH) in all the chromosomes of the three species. After silver staining, the nucleolar organizer regions (NORs) were located in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome rearrangements; Heterochromatin; Karyotype; NORs; FISH. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200012 |
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Leitao, Alexandra; Boudry, Pierre; Labat, Jean-philippe; Thiriot-quievreux, Catherine. |
Chromosomes of six cupped oyster species were studied using karyometric analysis alter conventional Giemsa staining, and silver staining, Karyotypes of Crassotrea angulata (nine metacentric and one submetacentric chromosome pairs), C. sikamea (nine metacentric and one submetacentric chromosome pairs), C. virginica (eight metacentric and two submetacentric chromosome pairs), C, ariakensis (eight metacentric and two submetacentric chromosome pairs), C, gasar (six metacentric and four submetacentric chromosome pairs), and Saccostrea commercialis (eight metacentric and two submetacentric chromosome pairs) are distinguishable by the number and position of the submetacentric chromosome pair and by the location of nucleolus organizer regions, Comparative... |
Tipo: Text |
Palavras-chave: Bivalvia; Cytotaxonomy; Karyotype; Chromosome; Cupped oyster. |
Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/publication-2737.pdf |
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Baeza,Carlos; Finot,Víctor L.; Ruiz,Eduardo. |
Alstroemeria L., one of the most diverse genera of the Chilean flora and of high floricultural value, is represented by 35 species, most of them distributed between 28–38° S in the Mediterranean zone of Central Chile. There are 24 complex-forming taxa, of which 18 have conservation problems (8 are considered “endangered” and 10 as “vulnerable”). One of these complexes is Alstroemeria presliana Herb. with two subspecies: subsp. presliana and subsp. australis Bayer. Alstroemeria presliana grows in Chile and Argentina: subsp. presliana is distributed from Reserva Nacional Siete Tazas (35°27′ S, Region of Maule) to Antuco, (37°25′ S, Region of Bío-Bío), and is also found in Neuquén, Argentina; subsp. australis is endemic to the Cordillera of Nahuelbuta. A... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alstroemeria; Asymmetry; Chile; Karyotype; Species complex. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200199 |
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Baeza,Carlos; Ruiz,Eduardo; Negritto,María. |
Alstroemeria L. (Alstroemeriaceae) is an American genus of monocots with two principal distribution centers in Chile and Brazil. In Chile, it is represented by about 32 species, most of them in central Chile, an area known for its high level of endemism. The "complex" Alstroemeria hookeri is endemic to Chile, where it is distributed from the Coquimbo to the Bío-Bío Region. We analyzed the karyotypes of 36 populations of this complex along its natural distribution. Ten metaphases per population were used for chromosome measurements. All analyzed subspecies presented a well defined asymmetric karyotype. The populations of A. hookeri subsp. hookeri collected in the coastal range of the Bío-Bío Region and the populations from the Central Valley of this Region... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alstroemeria hookeri; Complex; Karyotype; Polymorphisms; Chile. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100020 |
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Vicari,Marcelo Ricardo; Almeida,Mara Cristina de; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando; Artoni,Roberto Ferreira. |
We used differential staining techniques (BSG, GTG, AgNO3, DAPI and CMA3 banding) and fluorescent in situ hybridization (FISH) with 5S and 18S probes to investigated the karyotypic and cytogenetic chracteristics of Prochilodus lineatus specimens from a population in Vila Velha state park (Parque Estadual de Vila Velha, Ponta Grossa, Paraná state, southern Brazil). The specimens studied showed the same karyotype as that found in other P. lineatus populations, i.e. 2n = 54 biarmed chromosomes (40m + 14 sm) and c-positive heterochromatin preferentially located pericentromerically in all chromosomes. The presence of partial or totally heterochromatic supernumerary chromosomes with numeric intra-individual variation was confirmed in the analyzed population. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish cytogenetics; Prochilodus; Karyotype; Minor and major rDNA; NOR polymorphism. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400008 |
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Martinez,Emanuel Ricardo Monteiro; Nirchio,Mauro; Granado,Angel; Foresti,Fausto; Oliveira,Claudio. |
Cytogenetic analyses performed in Cephalosilurus apurensis, Microglanis aff. cottoides and Pseudopimelodus bufonius revealed that the three species have 2n = 54 chromosomes: C. apurensis presented six metacentric (M), 28 submetacentric (SM), 14 subtelolocentric (ST), and six acrocentric (A) chromosomes, while M. aff. cottoides showed 10M, 32SM, 10ST and 2A, and P. bufonius had 12M, 30SM and 12ST. The nucleolus organizer regions (NORs) were present on the short arm of a middle-sized ST pair, identified as pair 19, in C. apurensis NORs were found on the short arm of a middle-sized ST (pair 23) and on the long arm of a middle-sized ST (pair 22) in M. aff. cotttoides and on the short arm of three middle-sized ST pairs, identified as pairs 9, 10 and 11, in P.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Karyotype; Chromosomes; C-banding; Ag-NOR; Fish. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400015 |
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Medrado,Aline Souza; Figueiredo,Alba Vivian Amaral; Waldschmidt,Ana Maria; Affonso,Paulo Roberto Antunes de Mello; Carneiro,Paulo Luiz Souza. |
In the present work, morphometric and cytogenetic analyses were carried out in populations of the fish Astyanax fasciatus (Characidae) from Contas and Recôncavo Sul River basins (State of Bahia, Brazil), providing new data on the genetic structure of this species along the region. Based on morphologic measurements, we observed that populations from the same hydrographic basin were more similar to each other (Contas and Preto do Costa Rivers), and remarkably divergent from Recôncavo Sul (Mineiro Stream), as indicated by clustering analysis. Cytogenetic data revealed a same diploid number for all populations (2n = 48), but distinct karyotype formulae (8M+24SM+12ST+4A, FN = 92 in Contas River, 8M+24SM+10ST+6A, FN = 90 in Preto do Costa River, and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canonic variables; Karyotype; Morphometry; NOR. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200007 |
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Gunski,Ricardo José; Cunha,Isabel Souza; Degrandi,Tiago Marafiga; Ledesma,Mario; Garnero,Analía Del Valle. |
Podocnemis expansa and P. unifilis present 2n = 28 chromosomes, a diploid number similar to those observed in other species of the genus. The aim of this study was to characterize these two species using conventional staining and differential CBG-, GTG and Ag-NOR banding. We analyzed specimens of P. expansa and P. unifilis from the state of Tocantins (Brazil), in which we found a 2n = 28 and karyotypes differing in the morphology of the 13th pair, which was submetacentric in P. expansa and telocentric in P. unifilis. The CBG-banding patterns revealed a heterochromatic block in the short arm of pair 13 of P. expansa and an interstitial one in pair 13 of P. unifilis, suggesting a pericentric inversion. Pair 14 of P. unifilis showed an insterstitial band in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome banding; Ag-NORs; Karyotype; Chelonids. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300009 |
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Nirchio,Mauro; Rondón,Rodolfo; Oliveira,Claudio; Ferreira,Irani A.; Martins,Cesar; Pérez,Julio; Sola,Luciana; Rossi,Anna Rita. |
In the present study, three species of Lutjaninae, Lutjanus analis, L. griseus and L. synagris, were analyzed by conventional Giemsa staining, C-banding and silver staining, to reveal active Nucleolus Organizer Regions (NORs). Fluorescent in situ hybridization (FISH) was also applied to establish the number and location of the ribosomal gene clusters (18S and 5S rRNA genes). Counts of diploid metaphasic cells revealed a diploid modal chromosome complement composed of 48 acrocentric chromosomes in both L. analis and L. griseus. Two cytotypes were observed in L. synagris: cytotype I, with 2n=48 acrocentric chromosomes, found in 19 specimens, and cytotype II, with 46 acrocentric chromosomes and one large metacentric, found in two specimens. The large... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Karyotype; Ribosomal genes; NOR polymorphism; C-banding; Robertsonian rearrangement. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000100012 |
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Velloso,E.D.R.P.; Chauffaille,M.L.; Peliçario,L.M.; Tanizawa,R.S.S.; Toledo,S.R.C.; Gaiolla,R.D.; Lopes,L.F.. |
Myelodysplastic syndromes (MDS) and juvenile myelomonocytic leukemia (JMML) are rare hematopoietic stem cell diseases affecting children. Cytogenetics plays an important role in the diagnosis of these diseases. We report here the experience of the Cytogenetic Subcommittee of the Brazilian Cooperative Group on Pediatric Myelodysplastic Syndromes (BCG-MDS-PED). We analyzed 168 cytogenetic studies performed in 23 different cytogenetic centers; 84 of these studies were performed in patients with confirmed MDS (primary MDS, secondary MDS, JMML, and acute myeloid leukemia/MDS+Down syndrome). Clonal abnormalities were found in 36.9% of the MDS cases and cytogenetic studies were important for the detection of constitutional diseases and for differential diagnosis... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Childhood myelodysplastic syndromes; Juvenile myelomonocytic leukemia; Cytogenetics; Karyotype; Diagnosis. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100085 |
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Nogueira,Denise M.; Ferreira,Ana Maria R.; Goldschmidt,Beatriz; Pissinatti,Alcides; Carelli,Juliane B.; Verona,Carlos E.. |
In the Atlantic forest of Rio de Janeiro, Callithrix aurita (É. Geoffroy in Humboldt, 1812) is a native species vulnerable to extinction and C. jacchus (Linnaeus, 1758) and C. penicillata (É. Geoffroy, 1812) are invasive species. The major threats to the native species are habitat degradation and hybridization, although there are currently no genetic data about natural hybrids available. Previous studies have revealed that species of the Callithrix genus are extremely homogeneous in their karyotypes with the exceptions of the morphology and size of the Y chromosome and its nucleolar organizer region (NOR) banding pattern. Three male marmosets captured in the wild in Guapimirim municipality, Rio de Janeiro, Brazil, considered as possible hybrids between C.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Marmosets; Y-chromosome; Karyotype; Hybridization. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212011000200002 |
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Franco-de-Sá,Jorge Felipe Oliveira; Rosas,Fernando César Weber; Feldberg,Eliana. |
The giant otter, Pteronura brasiliensis Zimmermann 1780 (Carnivora, Mustelidae, Lutrinae), was widely distributed in South America but stable populations are now only found in the Pantanal and Amazon regions and the species is classified as endangered. There is only one recognized species of giant otter, although two subspecies of doubtful value have also been cited in the literature. We present the first karyotype of four captive P. brasiliensis specimens, all of which posses 2n = 38 chromosomes as 14M+8SM+6ST+8A and one pair of sexual chromosomes. An heteromorphic secondary constriction, associated with the nucleolar organizer region (NOR), was seen on the long arms of chromosome pair 17. The C-banding technique revealed heterochromatin in the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazon; Aquatic mammals; Ariranha; Karyotype. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600011 |
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Nirchio,Mauro; Cipriano,Roger; Cestari,Margarete; Fenocchio,Alberto. |
Karyotype of M. curema from the Gulf of Mexico and Brazil have been reported as possessing chromosome complement with 2n=28 and FN=48, whereas specimens from Venezuela has been reported as possessing a diploid number 2n=24 and a conserved FN (48). Although at first sight this variation suggests the presence of a chromosomal intraspecific (interpopulational) variability, the possibility that we are dealing with two different species was examined. This work revisit the karyotypes of M. curema from Venezuela and Brazil, including new data on C-banding, and NOR localization, and compares morphologic characteristics of samples from both localities. Thus, besides diploid number, the constitutive heterochromatin distribution and NORs location, mark other... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Karyotype; C-band; NOR-banding; Meristic and morphometric features; Species identification. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000100006 |
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SILVA,MONIQUE O.M.; ARMADA,JORGE LUÍS A. DE; VERONA,CARLOS EDUARDO S.; HELIODORO,GABRIELA; NOGUEIRA,DENISE M.. |
ABSTRACT The birth of fraternal twins is a characteristic frequently observed in callitrichids. Cytogenetic studies have demonstrated hematopoietic chimerism in marmosets with the occurrence of two cell lines 2n=46,XX/46,XY in females and males co-twins, without phenotypic changes. Amplification by PCR have also been used to verify the presence of the SRY gene in female chimaeras. Our aim was to verify the occurrence of chimerism in Callithrix sp. individuals considered as hybrids according to their intermediate phenotypes between C. jacchus and C. penicillata. Blood samples from 37 Callithrix sp. individuals were collected. Hematopoietic chimerism 2n=46,XX/46,XY was detected by cytogenetic analysis in five individuals, three males and two females. A... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Karyotype; SRY; Chimaeras; Hybridization. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602793 |
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Registros recuperados: 94 | |
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